Translational_Unit

Part:BBa_K4187028

Designed by: Martin Pezous   Group: iGEM22_Ionis_Paris   (2022-10-11)


pBAD-RBS-mtrC-T7Te-T1Te

mtrC is a membrane cytochrome c-based protein expressed natively in Shewanella oneidensis. It carries out a function of extracellular electron transfer and was shown to improve electron Transfer to the bacteria surroundings in Dundas CM, Walker DJF, Keitz BK. Tuning Extracellular Electron Transfer by Shewanella oneidensis Using Transcriptional Logic Gates. ACS Synth Biol. 2020 Sep 18. We used this part to try to improve the electric output of our microbial fuel cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3038
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 3038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3038
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3038
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1480
    Illegal SapI site found at 961


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Categories
Parameters
None